| Product Name | Fish Leukotriene B4 (LT-B4) ELISA Kit |
|---|---|
| Description | Leukotriene B4 is a leukotriene involved in inflammation. It is produced from leukocytes in response to inflammatory mediators and is able to induce the adhesion and activation of leukocytes on the endothelium, allowing them to bind to and cross it into the tissue. In neutrophils, it is also a potent chemoattractant, and is able to induce the formation of reactive oxygen species and the release of lysosome enzymes by these cells. Leukotriene B4 is a potent pro-inflammatory molecule that belongs to a family of eicosanoid lipid mediators. It is synthesized from arachidonic acid that is generated from nuclear membrane phospholipid. In general, and upon cell activation, phospholipase A2 is directed to the nuclear membrane. Here, it generates arachidonic acid that is bound by one 23 kDa membrane-bound protein termed Five-Lipooxygenase Activating Protein (FLAP). This assay has high sensitivity and excellent specificity for detection of Fish LT-B4. No significant cross-reactivity or interference between Fish LT-B4 and analogues was observed. |
| Method | Sandwich ELISA |
| Detection Range | 246.9-20000 pg/mL |
| Sensitivity | 89.3 pg/mL |
| Reactivity | Fish |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background |
Leukotriene B4 is a leukotriene involved in inflammation. It is produced from leukocytes in response to inflammatory mediators and is able to induce the adhesion and activation of leukocytes on the endothelium, allowing them to bind to and cross it into the tissue. In neutrophils, it is also a potent chemoattractant, and is able to induce the formation of reactive oxygen species and the release of lysosome enzymes by these cells. Leukotriene B4 is a potent pro-inflammatory molecule that belongs to a family of eicosanoid lipid mediators. It is synthesized from arachidonic acid that is generated from nuclear membrane phospholipid. In general, and upon cell activation, phospholipase A2 is directed to the nuclear membrane. Here, it generates arachidonic acid that is bound by one 23 kDa membrane-bound protein termed Five-Lipooxygenase Activating Protein (FLAP). |
| Supplier | Abebio |
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