Human 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1) ELISA Kit
| Product Name | Human 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1) ELISA Kit |
|---|---|
| Description | CYP7B1 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in CYP7B1 have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder. Human 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1) ELISA Kit employs a two-site sandwich ELISA to quantitate CYP7B1 in samples. An antibody specific for CYP7B1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP7B1 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human CYP7B1 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of CYP7B1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | CYP7B1, CBAS3, CP7B, SPG5A, cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1, oxysterol 7alpha-hydroxylase |
| Method | Sandwich ELISA |
| Detection Range | 15 ng/L-240 ng/L |
| Sensitivity | 1.0 ng/L |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 9420 |
| Storage | 2-8°C |
| Background | CYP7B1 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in CYP7B1 have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder. |
| Supplier | Abbkine |
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