Human Anti-deoxyribonuclease B antibody (DNase B-Ab) ELISA Kit
| Product Name | Human Anti-deoxyribonuclease B antibody (DNase B-Ab) ELISA Kit |
|---|---|
| Description | Human Anti-deoxyribonuclease B antibody (DNase B-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Human DNase B-Ab. No significant cross-reactivity or interference between Human DNase B-Ab and analogues was observed. DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. It has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis.DNase I binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. The function of this interaction is unclear. However since actin-bound DNase I is enzymatically inactive, the DNase-actin complex might be a storage form of DNase I that prevents damage of the genetic information. This Human Anti-deoxyribonuclease B antibody (DNase B-Ab) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNase B-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated DNase B-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled DNase B-Ab and unlabeled DNase B-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of DNase B-Ab in the sample. The color development is stopped and the intensity of the color is measured. |
| Synonyms | DNase B-Ab |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Background | DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. It has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis.DNase I binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. The function of this interaction is unclear. However since actin-bound DNase I is enzymatically inactive, the DNase-actin complex might be a storage form of DNase I that prevents damage of the genetic information. |
| Supplier | Abbkine |
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