| Product Name | Human Asialoglyco protein receptor (ASGPR) ELISA Kit |
|---|---|
| Description | Human Asialoglyco protein receptor (ASGPR) ELISA Kit has high sensitivity and excellent specificity for detection of Human ASGPR. No significant cross-reactivity or interference between Human ASGPR and analogues was observed. The asialoglycoprotein receptor binds to desialylated (galactosyl-terminal) glycoproteins. It transports these glycoproteins via a series of membrane vesicles and tubules to an acidic-sorting organelle where the receptor and ligand dissociate.The ASGR1 gene encodes a glycoprotein that forms the asialoglycoprotein receptor, also known as the Ashwell receptor, which is specific for desialylated (galactosyl-terminal) glycoproteins and is expressed exclusively in hepatic parenchymal cells. Partially deglycosylated plasma glycoproteins are efficiently and specifically removed from the circulation via this receptor-ligand complex. Many functional studies describing the kinetics of ligand-binding, internalization, and recycling of such receptors have been performed on the human hepatoma cell line HepG2. This Human Asialoglyco protein receptor (ASGPR) ELISA Kit employs a two-site sandwich ELISA to quantitate ASGPR in samples. An antibody specific for ASGPR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyASGPR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ASGPR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ASGPR bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | ASGPR |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Background | The asialoglycoprotein receptor binds to desialylated (galactosyl-terminal) glycoproteins. It transports these glycoproteins via a series of membrane vesicles and tubules to an acidic-sorting organelle where the receptor and ligand dissociate.The ASGR1 gene encodes a glycoprotein that forms the asialoglycoprotein receptor, also known as the Ashwell receptor, which is specific for desialylated (galactosyl-terminal) glycoproteins and is expressed exclusively in hepatic parenchymal cells. Partially deglycosylated plasma glycoproteins are efficiently and specifically removed from the circulation via this receptor-ligand complex. Many functional studies describing the kinetics of ligand-binding, internalization, and recycling of such receptors have been performed on the human hepatoma cell line HepG2. |
| Supplier | Abbkine |
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