| Product Name | Human ATP-dependent RNA helicase DDX25 (DDX25) ELISA Kit |
|---|---|
| Description | Human ATP-dependent RNA helicase DDX25 (DDX25) ELISA Kit has high sensitivity and excellent specificity for detection of Human DDX25. No significant cross-reactivity or interference between Human DDX25 and analogues was observed. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX25 encodes a member of this family. The encoded protein is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis. This Human ATP-dependent RNA helicase DDX25 (DDX25) ELISA Kit employs a two-site sandwich ELISA to quantitate DDX25 in samples. An antibody specific for DDX25 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDDX25 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DDX25 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DDX25 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | DDX25, GRTH, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25, gonadotropin-regulated testicular RNA helicase |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 29118 |
| Storage | 2-8°C |
| Background | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX25 encodes a member of this family. The encoded protein is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis. |
| Supplier | Abbkine |
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