Human Autoantibodies against the C-terminal domain 1 (ACAST-D1) ELISA Kit
| Product Name | Human Autoantibodies against the C-terminal domain 1 (ACAST-D1) ELISA Kit |
|---|---|
| Description | Human Autoantibodies against the C-terminal domain 1 (ACAST-D1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACAST-D1. No significant cross-reactivity or interference between Human ACAST-D1 and analogues was observed. The C-terminus (also known as the carboxyl-terminus, carboxy-terminus, C-terminal tail, C-terminal end, or COOH-terminus) is the end of an amino acid chain (protein or polypeptide), terminated by a free carboxyl group (-COOH). When the protein is translated from messenger RNA, it is created from N-terminus to C-terminus. The convention for writing peptide sequences is to put the C-terminal end on the right and write the sequence from N- to C-terminus. This Human Autoantibodies against the C-terminal domain 1 (ACAST-D1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACAST-D1 in samples. An antibody specific for ACAST-D1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACAST-D1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACAST-D1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACAST-D1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | ACAST-D1 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Background | The C-terminus (also known as the carboxyl-terminus, carboxy-terminus, C-terminal tail, C-terminal end, or COOH-terminus) is the end of an amino acid chain (protein or polypeptide), terminated by a free carboxyl group (-COOH). When the protein is translated from messenger RNA, it is created from N-terminus to C-terminus. The convention for writing peptide sequences is to put the C-terminal end on the right and write the sequence from N- to C-terminus. |
| Supplier | Abbkine |
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