| Product Name | Human Beta-melanocyte stimulating hormone (B-MSH) ELISA Kit |
|---|---|
| Description | Human Beta-melanocyte stimulating hormone (B-MSH) ELISA Kit has high sensitivity and excellent specificity for detection of Human B-MSH. No significant cross-reactivity or interference between Human B-MSH and analogues was observed. beta-MSH: a functional ligand that regulated energy homeostasis via hypothalamic MC4-R. beta-MSH is also capable of activating MC4-R and inhibiting feeding. beta-MSH acts as an endogenous MC4-R agonist and that this melanocortin peptide plays a role in the regulation of feeding and energy balance. Food-restriction significantly increased beta-MSH levels in the VMH, DMH and ARC above freely-fed controls, whilst alpha-MSH concentrations were unchanged. beta-MSH has higher affinity at MC4-R than alpha-MSH;beta-MSH activates GPCR in these sites, which are rich in MC4-R; beta-MSH is present in hypothalamic nuclei that regulate feeding and its concentrations alter with nutritional state. beta-MSH rather than alpha-MSH is the key ligand at the MC4-R populations that regulate feeding, and that inhibition of tonic release of beta-MSH is one mechanism contributing to hunger in under-feeding. This Human Beta-melanocyte stimulating hormone (B-MSH) ELISA Kit employs a two-site sandwich ELISA to quantitate B-MSH in samples. An antibody specific for B-MSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyB-MSH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for B-MSH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of B-MSH bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | B-MSH |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Background | beta-MSH: a functional ligand that regulated energy homeostasis via hypothalamic MC4-R. beta-MSH is also capable of activating MC4-R and inhibiting feeding. beta-MSH acts as an endogenous MC4-R agonist and that this melanocortin peptide plays a role in the regulation of feeding and energy balance. Food-restriction significantly increased beta-MSH levels in the VMH, DMH and ARC above freely-fed controls, whilst alpha-MSH concentrations were unchanged. beta-MSH has higher affinity at MC4-R than alpha-MSH;beta-MSH activates GPCR in these sites, which are rich in MC4-R; beta-MSH is present in hypothalamic nuclei that regulate feeding and its concentrations alter with nutritional state. beta-MSH rather than alpha-MSH is the key ligand at the MC4-R populations that regulate feeding, and that inhibition of tonic release of beta-MSH is one mechanism contributing to hunger in under-feeding. |
| Supplier | Abbkine |
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