| Product Name | Human Bridging integrator 2 (BIN2) ELISA Kit |
|---|---|
| Description | Human Bridging integrator 2 (BIN2) ELISA Kit has high sensitivity and excellent specificity for detection of Human BIN2. No significant cross-reactivity or interference between Human BIN2 and analogues was observed. BIN2 has acidic and serine/proline-rich stretches but lacks a C-terminal SH3 domain or a MYC-interacting region. Northern blot analysis revealed expression of a major 2.6-kb transcript that was highest in spleen and peripheral blood leukocytes and also high in thymus, colon, and placenta, suggesting preferential expression in hematopoietic tissues. Strong expression was detected in lymphoid and granulocytic cell lines but not other cell lines. Coimmunoprecipitation and Western blot analyses showed expression of an 80-kD protein that interacts with the N-terminal portion of the BAR domain of BIN1 isoforms but not with AMPH. Immunofluorescence microscopy demonstrated cytosolic expression and lack of receptor-mediated endocytic function for BIN2. Functional analysis showed that BIN2 lacks tumor suppressor features. This Human Bridging integrator 2 (BIN2) ELISA Kit employs a two-site sandwich ELISA to quantitate BIN2 in samples. An antibody specific for BIN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBIN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BIN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BIN2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | BIN2, BRAP-1, breast cancer associated protein BRAP1, bridging integrator-2 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 51411 |
| Storage | 2-8°C |
| Background | BIN2 has acidic and serine/proline-rich stretches but lacks a C-terminal SH3 domain or a MYC-interacting region. Northern blot analysis revealed expression of a major 2.6-kb transcript that was highest in spleen and peripheral blood leukocytes and also high in thymus, colon, and placenta, suggesting preferential expression in hematopoietic tissues. Strong expression was detected in lymphoid and granulocytic cell lines but not other cell lines. Coimmunoprecipitation and Western blot analyses showed expression of an 80-kD protein that interacts with the N-terminal portion of the BAR domain of BIN1 isoforms but not with AMPH. Immunofluorescence microscopy demonstrated cytosolic expression and lack of receptor-mediated endocytic function for BIN2. Functional analysis showed that BIN2 lacks tumor suppressor features. |
| Supplier | Abbkine |
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