| Product Name | Human Chymase (CMA1) ELISA Kit |
|---|---|
| Description | Human Chymase (CMA1) ELISA Kit has high sensitivity and excellent specificity for detection of Human CMA1. No significant cross-reactivity or interference between Human CMA1 and analogues was observed. CMA1 encodes a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and is thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Alternative splicing results in multiple variants. This Human Chymase (CMA1) ELISA Kit employs a two-site sandwich ELISA to quantitate CMA1 in samples. An antibody specific for CMA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCMA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CMA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CMA1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | CMA1, CYH, MCT1, MGC119890, MGC119891, chymase, chymase 1 preproprotein transcript E, chymase 1 preproprotein transcript I, chymase, heart, chymase, mast cell, mast cell protease I |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 1215 |
| Storage | 2-8°C |
| Background | CMA1 encodes a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and is thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Alternative splicing results in multiple variants. |
| Supplier | Abbkine |
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