| Product Name | Human Complement 1q (C1q) ELISA Kit |
|---|---|
| Description | Human Complement 1q (C1q) ELISA Kit has high sensitivity and excellent specificity for detection of Human C1QB. No significant cross-reactivity or interference between Human C1QB and analogues was observed. C1q is a 400 KDa protein formed from 18 peptide chains in 3 subunits of 6. Each 6 peptide subunit consists of a Y-shaped pair of triple peptide helices joined at the stem and ending in a globular non-helical head.C1q is alarge molecule made up of 18 polypeptide chain s(6 of A,6 of B,and 6 of C)held together by acombination of covalent(disulfide) and noncovalent bonds. When assembled,the C1q full molecule has two distinct regions:a globular head and a longtail.Most of the antibodies raised against C1q specific to the secondary structure of the assembled chains and are nonreactive to the amino acid sequence of each chain.The tightly bound individual polypeptide chains are difficult to separate from this complex,which requires long incubations under reducing conditions and high urea concentrations.Several proteins have globular domains similar to C1q. This Human Complement 1q (C1q) ELISA Kit employs a two-site sandwich ELISA to quantitate C1QB in samples. An antibody specific for C1QB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyC1QB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C1QB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C1QB bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | C1QB, Complement component 1, q subcomponent, beta polypeptide, complement component C1q, B chain, complement subcomponent C1q chain B |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 713 |
| Storage | 2-8°C |
| Background | C1q is a 400 KDa protein formed from 18 peptide chains in 3 subunits of 6. Each 6 peptide subunit consists of a Y-shaped pair of triple peptide helices joined at the stem and ending in a globular non-helical head.C1q is alarge molecule made up of 18 polypeptide chain s(6 of A,6 of B,and 6 of C)held together by acombination of covalent(disulfide) and noncovalent bonds. When assembled,the C1q full molecule has two distinct regions:a globular head and a longtail.Most of the antibodies raised against C1q specific to the secondary structure of the assembled chains and are nonreactive to the amino acid sequence of each chain.The tightly bound individual polypeptide chains are difficult to separate from this complex,which requires long incubations under reducing conditions and high urea concentrations.Several proteins have globular domains similar to C1q. |
| Supplier | Abbkine |
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