Human Killer cell immunoglobulin-like receptor 2DS4 (KIR2DS4) ELISA Kit
| Product Name | Human Killer cell immunoglobulin-like receptor 2DS4 (KIR2DS4) ELISA Kit |
|---|---|
| Description | Using primers known to amplify HLA-C-specific NK receptors to screen clones with NK triggering activity, Bottino et al. (1996) also isolated a KIR2DS4 cDNA, which they termed KKA3. Sequence analysis revealed that KIR2DS4 encodes a deduced 304-amino acid protein with an extracellular domain similar to that of KIR2D p58 receptors; however, in the transmembrane region, there is a charged lysine residue, and the cytoplasmic tail has only 39 amino acids with no ITIM (immunoreceptor tyrosine-based inhibitory motif). KIRs with short cytoplasmic tails are associated with NK cell triggering rather than inhibition. Immunoprecipitation analysis showed that KIR2DS4 is expressed in 2 forms, one of 35 to 45 kD and the other of 55 to 58 kD. Flow cytometry analysis showed that KIR2DS4, unlike KIR2DS1 and KIR2DS2, was expressed in only a minority of individuals tested. This assay has high sensitivity and excellent specificity for detection of Human KIR2DS4. No significant cross-reactivity or interference between Human KIR2DS4 and analogues was observed. |
| Synonyms | XXbac-BPG230H20.3, CD158I, KIR1D, KIR412, KKA3, MGC120019, MGC125315, MGC125317, NKAT8, KIR antigen 2DS4|killer inhibitory receptor 4-1-2|natural killer cell inhibitory receptor |
| Method | Sandwich ELISA |
| Detection Range | 1.56-100 ng/mL |
| Sensitivity | 0.66 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background | Using primers known to amplify HLA-C-specific NK receptors to screen clones with NK triggering activity, Bottino et al. (1996) also isolated a KIR2DS4 cDNA, which they termed KKA3. Sequence analysis revealed that KIR2DS4 encodes a deduced 304-amino acid protein with an extracellular domain similar to that of KIR2D p58 receptors; however, in the transmembrane region, there is a charged lysine residue, and the cytoplasmic tail has only 39 amino acids with no ITIM (immunoreceptor tyrosine-based inhibitory motif). KIRs with short cytoplasmic tails are associated with NK cell triggering rather than inhibition. Immunoprecipitation analysis showed that KIR2DS4 is expressed in 2 forms, one of 35 to 45 kD and the other of 55 to 58 kD. Flow cytometry analysis showed that KIR2DS4, unlike KIR2DS1 and KIR2DS2, was expressed in only a minority of individuals tested. |
| Supplier | Abebio |
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