| Product Name | Human MAPK/MAK/MRK overlapping kinase (RAGE) ELISA Kit |
|---|---|
| Description | Tumor antigens recognized by cytolytic T cells (CTLs) can be classified into 4 groups: shared tumor-specific antigens encoded by MAGE-type genes that are silent in most normal tissues except testis and expressed in many tumors; differentiation antigens that are also expressed in normal cells; antigens resulting from tumor-specific point mutations; and antigens overexpressed in tumor cells. By screening cells expressing both HLA-B7 and kidney tumor cell RNA with autologous CTLs, followed by anchored PCR analysis, Gaugler et al. (1996) isolated a cDNA encoding RAGE. RT-PCR analysis detected expression only in retina and in a number of tumor cell types. One putative RAGE open reading frame encodes a 40-amino acid protein and includes the peptide recognized by HLA-B7-restricted CTLs. This assay has high sensitivity and excellent specificity for detection of Human RAGE. No significant cross-reactivity or interference between Human RAGE and analogues was observed. |
| Synonyms | MOK, RAGE1, MAPK/MAK/MRK overlapping kinase|renal cell carcinoma antigen (MOK protein kinase) |
| Method | Sandwich ELISA |
| Detection Range | 0.156-10 ng/mL |
| Sensitivity | 0.062 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background | Tumor antigens recognized by cytolytic T cells (CTLs) can be classified into 4 groups: shared tumor-specific antigens encoded by MAGE-type genes that are silent in most normal tissues except testis and expressed in many tumors; differentiation antigens that are also expressed in normal cells; antigens resulting from tumor-specific point mutations; and antigens overexpressed in tumor cells. By screening cells expressing both HLA-B7 and kidney tumor cell RNA with autologous CTLs, followed by anchored PCR analysis, Gaugler et al. (1996) isolated a cDNA encoding RAGE. RT-PCR analysis detected expression only in retina and in a number of tumor cell types. One putative RAGE open reading frame encodes a 40-amino acid protein and includes the peptide recognized by HLA-B7-restricted CTLs. |
| Supplier | Abebio |
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