| Product Name | Human Methyl-CpG-binding domain protein 3 (MBD3) ELISA Kit |
|---|---|
| Description | DPEP3 encodes a membrane-bound glycoprotein from the family of dipeptidases (EC 3.4.13.19) involved in hydrolytic metabolism of various dipeptides, including penem and carbapenem beta-lactam antibiotics. This gene is located on chromosome 16 in a cluster with another member of this family, DPEP2. The deduced 493-amino acid mouse protein contains several residues conserved among MBDs, including 6 cysteines, 3 histidines, and a glutamic acid that is important for catalytic activity. Mouse and human MBD3 share 68% amino acid identity. Northern blot analysis detected an Mbd2 RNA in mouse testis only. Phospholipase treatment released Mbd3 from transfected COS-7 cell membranes, indicating that Mbd3 is anchored to the membrane through glycosylphosphatidylinositol modification. This assay has high sensitivity and excellent specificity for detection of Human MBD3. No significant cross-reactivity or interference between Human MBD3 and analogues was observed. |
| Method | Sandwich ELISA |
| Detection Range | 0.312-20 ng/mL |
| Sensitivity | 0.105 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background | DPEP3 encodes a membrane-bound glycoprotein from the family of dipeptidases (EC 3.4.13.19) involved in hydrolytic metabolism of various dipeptides, including penem and carbapenem beta-lactam antibiotics. This gene is located on chromosome 16 in a cluster with another member of this family, DPEP2. The deduced 493-amino acid mouse protein contains several residues conserved among MBDs, including 6 cysteines, 3 histidines, and a glutamic acid that is important for catalytic activity. Mouse and human MBD3 share 68% amino acid identity. Northern blot analysis detected an Mbd2 RNA in mouse testis only. Phospholipase treatment released Mbd3 from transfected COS-7 cell membranes, indicating that Mbd3 is anchored to the membrane through glycosylphosphatidylinositol modification. |
| Supplier | Abebio |
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