| Product Name | Human Mitochondrial fission 1 protein (FIS1) ELISA Kit |
|---|---|
| Description | The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission. The deduced 152-amino acid protein contains a central leucine zipper, a coiled-coil region, and a C-terminal transmembrane domain. It also contains a region showing high interspecies homology and a putative tetratricopeptide repeat. Immunocytochemical analysis of HeLa cells detected endogenous FIS1 expression in mitochondria. Protease treatment indicated that FIS1 is expressed at the outer mitochondrial membrane. Transfection of a C-terminal truncated FIS1 resulted in cytoplasmic expression, indicating that the C terminus is required for mitochondrial localization. This assay has high sensitivity and excellent specificity for detection of Human FIS1. No significant cross-reactivity or interference between Human FIS1 and analogues was observed. |
| Synonyms | CGI-135, TTC11, H_NH0132A01.6|tetratricopeptide repeat domain 11 |
| Method | Sandwich ELISA |
| Detection Range | 0.312-20 ng/mL |
| Sensitivity | 0.115 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background |
The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission. The deduced 152-amino acid protein contains a central leucine zipper, a coiled-coil region, and a C-terminal transmembrane domain. It also contains a region showing high interspecies homology and a putative tetratricopeptide repeat. Immunocytochemical analysis of HeLa cells detected endogenous FIS1 expression in mitochondria. Protease treatment indicated that FIS1 is expressed at the outer mitochondrial membrane. Transfection of a C-terminal truncated FIS1 resulted in cytoplasmic expression, indicating that the C terminus is required for mitochondrial localization. |
| Supplier | Abebio |
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