| Product Name | Human PDZ and LIM domain protein 1 (PDLIM1) ELISA Kit |
|---|---|
| Description | The LIM domain of PDLIM1 interacts with the C-terminal EF-hand region of alpha-actinin-2 (ACTN2). Immunoprecipitation, Western blot analysis, and immunofluorescence microscopy demonstrated that the 36-kD PDLIM1 protein colocalizes with ACTN2 in the Z discs of myocardial sarcomeres, particularly at intercalated discs, and with vinculin (VCL), which is localized in the fascia adherens of intercalated discs. Sequence analysis predicted that the 329-amino acid PDLIM1 protein, which is 88% homologous to the rat protein, shares 51 to 61% identity with the PDZ domains of ENIGMA, ALP, ENH, and RIL. SDS-PAGE analysis showed that recombinant PDLIM1 was expressed as a 36-kD protein. This assay has high sensitivity and excellent specificity for detection of Human PDLIM1. No significant cross-reactivity or interference between Human PDLIM1 and analogues was observed. |
| Synonyms | CLIM1, CLP-36, CLP36, hCLIM1, carboxy terminal LIM domain protein 1|carboxyl terminal LIM domain protein 1|elfin|enigma |
| Method | Sandwich ELISA |
| Detection Range | 0.156-10 ng/mL |
| Sensitivity | 0.061 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background |
The LIM domain of PDLIM1 interacts with the C-terminal EF-hand region of alpha-actinin-2 (ACTN2). Immunoprecipitation, Western blot analysis, and immunofluorescence microscopy demonstrated that the 36-kD PDLIM1 protein colocalizes with ACTN2 in the Z discs of myocardial sarcomeres, particularly at intercalated discs, and with vinculin (VCL), which is localized in the fascia adherens of intercalated discs. Sequence analysis predicted that the 329-amino acid PDLIM1 protein, which is 88% homologous to the rat protein, shares 51 to 61% identity with the PDZ domains of ENIGMA, ALP, ENH, and RIL. SDS-PAGE analysis showed that recombinant PDLIM1 was expressed as a 36-kD protein. |
| Supplier | Abebio |
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