| Product Name | Human Perforin-1 (PRF1/PFP) ELISA Kit |
|---|---|
| Description | Perforin is a cytolytic protein found in the granules of CD8 T-cells and NK cells. Perforin is a cytolytic protein found in the granules of Cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. This assay has high sensitivity and excellent specificity for detection of Human PRF1. No significant cross-reactivity or interference between Human PRF1 and analogues was observed. |
| Synonyms | RP11-710A11.3, FLH2, HPLH2, MGC65093, P1, PFN1, PFP, OTTHUMP00000019759|cytolysin|lymphocyte pore forming protein|perforin 1 |
| Method | Sandwich ELISA |
| Detection Range | 1.56-100 ng/mL |
| Sensitivity | 0.62 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background | Perforin is a cytolytic protein found in the granules of CD8 T-cells and NK cells. Perforin is a cytolytic protein found in the granules of Cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. |
| Supplier | Abebio |
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