| Product Name | Human Phosphatase and actin regulator 1 (PHACTR1) ELISA Kit |
|---|---|
| Description | The PHACTR proteins share highest similarity in sequences surrounding the N- and C-terminal RPEL repeats and in the C-terminal actin- and PP1-binding domains. In situ hybridization of rat brain showed enrichment of Phactr1 in cerebral cortex, hippocampus, olfactory tubercle, nucleus accumbens, caudate-putamen, and piriform cortex. Endogenous PP1 coprecipitated with ectopically expressed rat Phactr1 in human embryonic kidney cells. Phactr1 also coprecipitated with actin in rat brain lysates. Yeast 2-hybrid analysis of a series of Phactr1 C-terminal truncation mutants indicated that PP1 bound to Phactr1 near the C terminus, while actin bound in a region containing RPEL repeats 2 and 3. This assay has high sensitivity and excellent specificity for detection of Human PHACTR1. No significant cross-reactivity or interference between Human PHACTR1 and analogues was observed. |
| Synonyms | RP1-257A7.1, KIAA1733, MGC126575, MGC126577, RPEL, RPEL1, dJ257A7.2, RPEL repeat containing 1 |
| Method | Sandwich ELISA |
| Detection Range | 23.44-1500 pg/mL |
| Sensitivity | 5.86 pg/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background |
The PHACTR proteins share highest similarity in sequences surrounding the N- and C-terminal RPEL repeats and in the C-terminal actin- and PP1-binding domains. In situ hybridization of rat brain showed enrichment of Phactr1 in cerebral cortex, hippocampus, olfactory tubercle, nucleus accumbens, caudate-putamen, and piriform cortex. Endogenous PP1 coprecipitated with ectopically expressed rat Phactr1 in human embryonic kidney cells. Phactr1 also coprecipitated with actin in rat brain lysates. Yeast 2-hybrid analysis of a series of Phactr1 C-terminal truncation mutants indicated that PP1 bound to Phactr1 near the C terminus, while actin bound in a region containing RPEL repeats 2 and 3. |
| Supplier | Abebio |
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