Human Phosphopantothenoylcysteine decarboxylase (PPCDC) ELISA Kit
| Product Name | Human Phosphopantothenoylcysteine decarboxylase (PPCDC) ELISA Kit |
|---|---|
| Description | Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC (EC 4.1.1.36), one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine. PPCDC functioned within the CoA synthetic pathway, and they verified PPCDC function by complementation in E. coli. Incubation of PPCS, PPCDC, and the bifunctional enzyme COASY with the necessary substrates and cofactors reconstituted the 4-step biochemical transformation of phosphopantothenate to CoA. Saccharomyces cerevisiae_Hal3 and Vhs3 are moonlighting proteins, forming an atypical heterotrimeric decarboxylase (PPCDC) required for CoA biosynthesis, and regulating cation homeostasis by inhibition of the Ppz1 phosphatase. This assay has high sensitivity and excellent specificity for detection of Human PPCDC. No significant cross-reactivity or interference between Human PPCDC and analogues was observed. |
| Synonyms | FLJ14585, MDS018, |
| Method | Sandwich ELISA |
| Detection Range | Request Information |
| Sensitivity | Request Information |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background | Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC (EC 4.1.1.36), one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine. PPCDC functioned within the CoA synthetic pathway, and they verified PPCDC function by complementation in E. coli. Incubation of PPCS, PPCDC, and the bifunctional enzyme COASY with the necessary substrates and cofactors reconstituted the 4-step biochemical transformation of phosphopantothenate to CoA. Saccharomyces cerevisiae_Hal3 and Vhs3 are moonlighting proteins, forming an atypical heterotrimeric decarboxylase (PPCDC) required for CoA biosynthesis, and regulating cation homeostasis by inhibition of the Ppz1 phosphatase. |
| Supplier | Abebio |
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