| Product Name | Human Probable arylformamidase (AFMID) ELISA Kit |
|---|---|
| Description | Arylformamidase (AFMID) is the second enzyme of the kynurenine pathway metabolizing tryptophan to nicotinic acid and nicotinamide adenine dinucleotide cofactors. Inhibition of AFMID by organophosphorus insecticides in developing chicken embryos is correlated with lowered NAD levels and severe teratogenesis. The cDNA sequence previously identified for mouse liver AFMID was cloned and expressed in Escherichia coli. Residues identified as potential catalytic triad members through sequence motif and homology modeling were mutated to alanine to probe their contributions to enzyme activity. The wild-type and mutant AFMIDs were expressed as amino terminal 6 x His-tagged recombinant proteins to facilitate purification. Three chromatography steps isolated highly purified proteins for enzyme activity comparisons. This assay has high sensitivity and excellent specificity for detection of Human AFMID. No significant cross-reactivity or interference between Human AFMID and analogues was observed. |
| Synonyms | DKFZp686F03259, KF, MGC167063, |
| Method | Sandwich ELISA |
| Detection Range | 1.56-100 ng/mL |
| Sensitivity | 0.57 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background |
Arylformamidase (AFMID) is the second enzyme of the kynurenine pathway metabolizing tryptophan to nicotinic acid and nicotinamide adenine dinucleotide cofactors. Inhibition of AFMID by organophosphorus insecticides in developing chicken embryos is correlated with lowered NAD levels and severe teratogenesis. The cDNA sequence previously identified for mouse liver AFMID was cloned and expressed in Escherichia coli. Residues identified as potential catalytic triad members through sequence motif and homology modeling were mutated to alanine to probe their contributions to enzyme activity. The wild-type and mutant AFMIDs were expressed as amino terminal 6 x His-tagged recombinant proteins to facilitate purification. Three chromatography steps isolated highly purified proteins for enzyme activity comparisons. |
| Supplier | Abebio |
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