| Product Name | Human Prolyl 4-hydroxylase subunit alpha-3 (P4HA3) ELISA Kit |
|---|---|
| Description | P4HA3 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. P4HA3 has all residues necessary to bind the peptide substrate, to coordinate Fe(2+) and the 2-oxoglutarate cosubstrate, and for enzymatic activity. Processed P4HA3 contains 525 amino acids and shares 91% and 94% amino acid identity with rat and mouse P4ha3, respectively, and 35% and 37% identity with P4HA1 and P4HA2, respectively. Northern blot analysis detected a 2.7-kb P4HA3 transcript. Highest expression was in placenta, adult liver, and fetal skin, with lower levels in fetal liver, lung, and muscle. RT-PCR detected P4HA3 expression in all tissues examined, with highest expression in placenta and fetal kidney, liver, and lung. Expression of P4HA3 was much lower than that of P4HA1. This assay has high sensitivity and excellent specificity for detection of Human P4HA3. No significant cross-reactivity or interference between Human P4HA3 and analogues was observed. |
| Synonyms | C-P4H alpha III|collagen prolyl 4-hydroxylase alpha(III)|procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III|prolyl 4-hydroxylase, alpha III subunit |
| Method | Sandwich ELISA |
| Detection Range | 0.156-10 ng/mL |
| Sensitivity | 0.066 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background | P4HA3 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. P4HA3 has all residues necessary to bind the peptide substrate, to coordinate Fe(2+) and the 2-oxoglutarate cosubstrate, and for enzymatic activity. Processed P4HA3 contains 525 amino acids and shares 91% and 94% amino acid identity with rat and mouse P4ha3, respectively, and 35% and 37% identity with P4HA1 and P4HA2, respectively. Northern blot analysis detected a 2.7-kb P4HA3 transcript. Highest expression was in placenta, adult liver, and fetal skin, with lower levels in fetal liver, lung, and muscle. RT-PCR detected P4HA3 expression in all tissues examined, with highest expression in placenta and fetal kidney, liver, and lung. Expression of P4HA3 was much lower than that of P4HA1. |
| Supplier | Abebio |
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