| Product Name | Human Ski-like protein (SKIL) ELISA Kit |
|---|---|
| Description | The mouse Sno gene expresses 2 isoforms, SnoN and SnoN2, that are different from each other in a location downstream of the site of alternative splicing previously described in the human SNO gene. SnoN2 is missing a 138-bp coding segment present in mouse SnoN and human SNON. TGFB induces activation and nuclear translocation of SMAD2, SMAD3, and SMAD4. SMAD3 causes degradation of SnoN, allowing a SMAD2/SMAD4 complex to activate TGFB target genes. To initiate a negative feedback mechanism that permits a precise and timely regulation of TGFB signaling, TGFB also induces an increased expression of SnoN at a later stage, which in turn binds to SMAD heteromeric complexes and shuts off TGFB signaling. This assay has high sensitivity and excellent specificity for detection of Human SKIL. No significant cross-reactivity or interference between Human SKIL and analogues was observed. |
| Synonyms | SNO, SnoA, SnoI, SnoN, SKI-like|ski-related oncogene snoN |
| Method | Sandwich ELISA |
| Detection Range | 31.25-2000 pg/mL |
| Sensitivity | 12.8 pg/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background | The mouse Sno gene expresses 2 isoforms, SnoN and SnoN2, that are different from each other in a location downstream of the site of alternative splicing previously described in the human SNO gene. SnoN2 is missing a 138-bp coding segment present in mouse SnoN and human SNON. TGFB induces activation and nuclear translocation of SMAD2, SMAD3, and SMAD4. SMAD3 causes degradation of SnoN, allowing a SMAD2/SMAD4 complex to activate TGFB target genes. To initiate a negative feedback mechanism that permits a precise and timely regulation of TGFB signaling, TGFB also induces an increased expression of SnoN at a later stage, which in turn binds to SMAD heteromeric complexes and shuts off TGFB signaling. |
| Supplier | Abebio |
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