| Product Name | Human Sorcin (SRI) ELISA Kit |
|---|---|
| Description | The 22-kD sorcin protein was overexpressed in HOB1/VCR-1.0 cells, a multidrug-resistant human B immunoblastic lymphoma cell line. By PCR with primers based on the sequence of hamster sorcin, the authors recovered a partial human sorcin cDNA. Using the partial cDNA to screen a HOB1/VCR-1.0 library, they isolated cDNAs corresponding to the entire sorcin coding region. The predicted 198-amino acid human protein shares 95% sequence identity with hamster sorcin. Human sorcin contains 4 EF-hand calcium-binding motifs. Purified protein bound calcium in vitro. Southern and Northern blot analysis revealed that the sorcin gene is amplified and overexpressed in HOB1/VCR-1.0 cells. No expression was detected in parental HOB1 cells. This assay has high sensitivity and excellent specificity for detection of Human SRI. No significant cross-reactivity or interference between Human SRI and analogues was observed. |
| Synonyms | FLJ26259, SCN, H_RG167B05.1|calcium binding protein amplified in mutlidrug-resistant cells |
| Method | Sandwich ELISA |
| Detection Range | 0.312-20 ng/mL |
| Sensitivity | 0.119 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background |
The 22-kD sorcin protein was overexpressed in HOB1/VCR-1.0 cells, a multidrug-resistant human B immunoblastic lymphoma cell line. By PCR with primers based on the sequence of hamster sorcin, the authors recovered a partial human sorcin cDNA. Using the partial cDNA to screen a HOB1/VCR-1.0 library, they isolated cDNAs corresponding to the entire sorcin coding region. The predicted 198-amino acid human protein shares 95% sequence identity with hamster sorcin. Human sorcin contains 4 EF-hand calcium-binding motifs. Purified protein bound calcium in vitro. Southern and Northern blot analysis revealed that the sorcin gene is amplified and overexpressed in HOB1/VCR-1.0 cells. No expression was detected in parental HOB1 cells. |
| Supplier | Abebio |
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