| Product Name | Syntaphilin, 1-424aa, Human, Recombinant |
|---|---|
| Description | Syntaphilin is a neuron-specific protein initially characterized as a binding partner of syntaxin-1. The protein competes with SNAP-25 for binding to syntaxin-1 and prevents the formation of the SNARE core complex, thereby controlling free syntaxin-1 availability for the assembly of the SNARE complex and potentially regulating synaptic vesicle exocytosis. Recombinant Syntaphilin protein was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | SNPH, Syntaphilin, KIAA0374. |
| Host | E. coli |
| Molecular Weight | 14.2 kDa (134 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MAMSLPGSRR TSAGSRRRTS PPVSVRDAYG TSSLSSSSNS GSYKGSDSSP TPRRSMKYTL CSDNHGIKPP TPEQYLTPLQ QKEVCIRHLK ARLKDTQDRL QDRDTEIDDL KTQLSRMQED WIEEECHRVE AQLALKEARK EIKQLKQVID TVKNNLIDKD KGLQKYFVDI NIQNKKLETL LHSMEVAQNG MAKEDGTGES AGGSPARSLT RSSTYTKLSD PAVCGDRQPG DPSSGSAEDG ADSGFAAADD TLSRTDALEA SSLLSSGVDC GTEETSLHSS FGLGPRFPAS NTYEKLLCGM EAGVQASCMQ ERAIQTDFVQ YQPDLDTILE KVTQAQVCGT DPESGDRCPE LDAHPSGPRD PNSAVVVTVG DELEAPEPIT RGPTPQRPGA NPNPGQSVSV VCPMEEEEEA AVAEKEPKSY WSRH |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl buffer (pH 7.5) containing 0.1 M NaCl,1mM MgCl2 |
| Concentration | 1 mg/ml (determined by BCA assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Lao G., et al. Neuron. 2000 25(1), 191-201. Boczan., et al. J Biol Chem. 2004 279(18), 18911-9. |
| Background | Syntaphilin is a neuron-specific protein initially characterized as a binding partner of syntaxin-1. The protein competes with SNAP-25 for binding to syntaxin-1 and prevents the formation of the SNARE core complex, thereby controlling free syntaxin-1 availability for the assembly of the SNARE complex and potentially regulating synaptic vesicle exocytosis. Recombinant Syntaphilin protein was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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