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|Product Name||TAF9, 1-172aa, Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MLLPNILLTG TPGVGKTTLG KELASKSGLK YINVGDLARE EQLYDGYDEE YDCPILDEDR VVDELDNQMR EGGVIVDYHG CDFFPERWFH IVFVLRTDTN VLYERLETRG YNEKKLTDNI QCEIFQVLYE EATASYKEEI VHQLPSNKPE ELENNVDQIL KWIEQWIKDH NS|
|Background||TAF9, also known as TATA box binding protein (TBP)-associated factor, is a general transcription factor that facilitates the preinitiation complex assembly through direct interactions with the TATA promoter element. It is a multisubunit complex consisting of a small TATA-binding polypeptide and other TBP-associated factors (TAFs). It acts as a channel for regulatory signals. Recombinant human TAF9 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol, 50mM NaCl|
|Intended Use||For Research Use Only|
|Molecular Weight||17.4 kDa (154aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)|
|NCBI Accession #||NP_057367|
|Purity||> 90% by SDS-PAGE|
|References||Matsui T, et al. (1980), J. Biol. Chem., 255: 11992-11996.
Dynlacht B.D., et al. (1991). Cell. 66:563-576.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||Adenylate kinase isoenzyme 6, AD-004, AK6, CGI-137, CINAP, CIP, hCINAP, MGC1603, MGC3647, MGC5067|
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