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|Product Name||TAGLN, 1-201aa, Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MANKGPSYGM SREVQSKIEK KYDEELEERL VEWIIVQCGP DVGRPDRGRL GFQVWLKNGV ILSKLVNSLY PDGSKPVKVP ENPPSMVFKQ MEQVAQFLKA AEDYGVIKTD MFQTVDLFEG KDMAAVQRTL MALGSLAVTK NDGHYRGDPN WFMKKAQEHK REFTESQLQE GKHVIGLQMG SNRGASQAGM TGYGRPRQII S|
|Background||TAGLN is a transformation and shape-change sensitive actin cross-linking/gelling protein that belongs to the calponin family. This protein is expressed abundantly in fibroblasts and smooth muscle. It is involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. During embryogenesis, TAGLN is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. It is downregulated in several transformed cell lines, indicating that a reduction of TAGLN expression may be an early indicator of the onset of transformation. Recombinant human TAGLN protein, fused to his-tag at N-terminus was expressed in E.coli and purified by using conventional chromatography techniques|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20 mM Tris-HCl Buffer (pH 7.5) containing 10% Glycerol|
|Intended Use||For Research Use Only|
|Molecular Weight||24.7 kDa(216aa), confirmed by MALDI-TOF|
|NCBI Accession #||NP_001001522|
|Purity||> 85 % by SDS-PAGE|
|References||Kobayashi R., et al. (1994) Biochem Biophys Res Commun. 198(3):1275-80.
Thweatt R., et al. (1992) Biochem Biophys Res Commun. 187(1):1-7.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||SM22, SMCC, TAGLN1, WS3-10, Transgelin, SM 22, SM22 alpha, SMCC, Smooth muscle protein 22-alpha, WS3 10|
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