TAT ELISA kit (Human)

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SEA831Hu
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Product Name TAT ELISA kit (Human)
Description This assay has high sensitivity and excellent specificity for detection of Thrombin/Antithrombin Complex (TAT). No significant cross-reactivity or interference between Thrombin/Antithrombin Complex (TAT) and analogues was observed.
Method Sandwich
Detection Range 6.25-400pg/mL
Sensitivity 3.09pg/mL
Target TAT
Specificity This assay has high sensitivity and excellent specificity for detection of Thrombin/Antithrombin Complex (TAT). No significant cross-reactivity or interference between Thrombin/Antithrombin Complex (TAT) and analogues was observed.
Reactivity Human
Sample Types Plasma
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot P00734 & P01008
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References High sCD40L levels early after trauma are associated with enhanced shock, sympathoadrenal activation, tissue and endothelial damage, coagulopathy and mortality;Prolonged Exposure to Sphingosine 1–Phosphate Receptor-1 Agonists Exacerbates Vascular Leak, Fibrosis, and Mortality after Lung Injury;Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India;Selected hemostatic parameters in patients with pancreatic tumors.;TFPI1 Mediates Resistance to Doxorubicin in Breast Cancer Cells by Inducing a Hypoxic-Like Response;Activated TAFI Promotes the Development of Chronic Thromboembolic Pulmonary Hypertension: A Possible Novel Therapeutic Target;The circadian rhythm of selected parameters of the hemostasis system in healthy people;
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