Testo ELISA kit (General species)

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Product Name Testo ELISA kit (General species)
Description This assay has high sensitivity and excellent specificity for detection of Testosterone (Testo). No significant cross-reactivity or interference between Testosterone (Testo) and analogues was observed.
Synonyms T; Testesteron; Testeronun
Method Competitive
Detection Range 370.4-30,000pg/mL
Sensitivity 152.5pg/mL
Target Testo
Specificity This assay has high sensitivity and excellent specificity for detection of Testosterone (Testo). No significant cross-reactivity or interference between Testosterone (Testo) and analogues was observed.
Sample Types Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately.
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Androgen Deprivation Induces Bladder Histological Abnormalities and Dysfunction via TGF-β in Orchiectomized Mature Rats;Insulin Receptor Substrate 2 Is Required for Testicular Development;Central effects of camphor on GnRH and sexual hormones in male rat;Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats;The effect of sertraline, paroxetine, fluoxetine and escitalopram on testicular tissue and oxidative stress parameters in rats.;Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering;Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis;Diets rich in saturated and polyunsaturated fatty acids induce morphological alterations in the rat ventral prostate;Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring;The impact of maternal consumption of cafeteria diet on reproductive function in the offspring;The effect of Sertraline, Paroxetine, Fluoxetine and Escitalopram on testicular tissue and oxidative stress parameters in rats;Perfluorooctane sulfonate effects on the reproductive axis in adult male rats;Lard and/or canola oil-rich diets induce penile morphological alterations in a rat model.;Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility;Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy;Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes;Metabolic and Testicular Effects of the Long-Term Administration of Different High-Fat Diets in Adult Rats;Plasma Testosterone and Testis Histological Features of Mice Treated with Averrhoa Bilimbi, Cosmos Caudatus and Pereskia Bleo Ethanolic Extract;Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy;Effect on the H20 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis period;Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarction;Preparation and evaluation of a phospholipid-based injectable gel for the long term delivery of leuprolide acetaterrh;Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota;Expression of androgen-producing enzyme genes and testosterone concentration in AnCavia (Guinea pig )s and Nellore heifers with high and low ovarian follicle count;Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARα;THE EFFECT OF KISSPEPTIN ON SPERMATOGENESIS AND APOPTOSIS IN RATS;Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice;Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus;Maternal Sleep Deprivation Alters Reproductive Capability of Male Offspring in Wistar Rats;Recovery of fertility in quinestrol-treated or diethylstilbestrol-treated mice: Implications for rodent management.;Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.;Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum.;Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα;Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats.;The effect of kisspeptin on spermatogenesis and apoptosis in rats.;The Angiogenic Effect of Testosterone Supplementation on Brain of Aged Mice.;Abnormality of Klotho Signaling Is Involved in Polycystic Ovary Syndrome;Testosterone deficiency prevents left ventricular contractility dysfunction after myocardial infarction ;The effect of testosterone supplementation on morphometry and VEGF expression level of thoracic aorta in aged mice;Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob -/-);Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway;Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population;Exposure to tris (1, 3-dichloro-2-propyl) phosphate for Two generations decreases fecundity of zebrafish at environmentally relevant concentrations;deficiency leads to testicular dysfunction and altered expression of genes involved in cell communication, chromatin reorganization, spermatogenesis, and …;DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway;Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4) …;Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes …;Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons;Resveratrol attenuates metabolic, sperm, and testicular changes in adult Wistar rats fed a diet rich in lipids and simple carbohydrates;;Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats;Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients;Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome;Brain‐derived neurotrophic factor: A steroidogenic regulator of Leydig cells;Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary;iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine;The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study.;The Effect of Testosterone Supplementation on Capillar Morphometry and VEGF Expression Level of the Heart in Aged Mice;Cyclophilin D participates in the inhibitory effect of high‐fat diet on the expression of steroidogenic acute regulatory protein;Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male rats;Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling;Effects of fast food packaging plasticizers and their metabolites on steroid hormone synthesis in H295R cells;Serum Progesterone and Testosterone Levels in Schizophrenia Patients at Different Stages of Treatment;Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance …;Novel antihypertensive action of rutin is mediated via inhibition of angiotensin converting enzyme/mineralocorticoid receptor/angiotensin 2 type 1 receptor (ATR1) …;Parental exposure to polystyrene microplastics at environmentally relevant concentrations has negligible transgenerational effects on zebrafish (Danio rerio);MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance;Electroacupuncture alleviates polycystic ovary syndrome-like symptoms through improving insulin resistance, mitochondrial dysfunction, and endoplasmic …;Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal Model;Maternal CBZ exposure impairs testicular development, spermatogenesis and sperm parameters in male offspring at puberty;N, N-Dimethylacetamide, an FDA approved excipient, acts post-meiotically to impair spermatogenesis and cause infertility in rats;Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress;Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS;IVERMECTINA IMPEDE DANOS TESTICULARES EM RATOS;The effects of gold nanoparticles on Leydig cells and male reproductive function in mice;The Effect of Acupuncture on MiRNA Expression Profiles in the Ovaries of Rats with PCOS and Insulin Resistance;BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-¦ B pathways in aging mice induced by D-galactose;Exposure to the bromodomain inhibitor N-methyl pyrrolidone blocks spermatogenesis in a hormonal and non-hormonal fashion;A comparative study on the aphrodisiac activity of food plants Mondia whitei, Chenopodium album, Ccucurbita pe...;MiR-1224-5p attenuates polycystic ovary syndrome through inhibiting NOD-like receptor protein 3 inflammasome activation via targeting Forkhead box O 1;Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice;Yohimbine hydrochloride inhibits benign prostatic hyperplasia by downregulating steroid 5α-reductase type 2;Superior effect of broccoli methanolic extract on control of oxidative damage of sperm cryopreservation and reproductive performance in rats: A comparison with …;Cylindrospermopsin Disrupts Estrous Cycle and Increases Spermatogenesis in Mice;
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