TFF1 ELISA kit (Human)

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SEB049Hu
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Product Name TFF1 ELISA kit (Human)
Description This assay has high sensitivity and excellent specificity for detection of Trefoil Factor 1 (TFF1). No significant cross-reactivity or interference between Trefoil Factor 1 (TFF1) and analogues was observed.
Synonyms BCEI; HP1.A; HPS2; PS2; PNR2; Breast Cancer,Estrogen-Inducible Sequence Expressed In; Breast cancer estrogen-inducible protein; Polypeptide P1.A
Method Sandwich
Detection Range 0.156-10ng/mL
Sensitivity 0.056ng/mL
Target TFF1
Specificity This assay has high sensitivity and excellent specificity for detection of Trefoil Factor 1 (TFF1). No significant cross-reactivity or interference between Trefoil Factor 1 (TFF1) and analogues was observed.
Reactivity Human
Sample Types Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot P04155
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Serum trefoil factor 3 is a promising non-invasive biomarker for gastric cancer screening: A monocentric cohort study in China;miR218-5p regulates the proliferation of gastric cancer cells by targeting TFF1 in an Erk1/2-dependent manner;Identification of a Three-Biomarker Panel in Urine for Early Detection of Pancreatic Adenocarcinoma;miR-632 promotes gastric cancer progression by accelerating angiogenesis in a TFF1-dependent manner;Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid …;A combination of urinary biomarker panel and PancRISK score for earlier detection of pancreatic cancer: A case¨Ccontrol study;Untersuchungen der Trefoil factor family Peptide 1-3 in humaner Synovialfl¨¹ssigkeit und Synovialmembran und deren Bedeutung bei Arthrose und?¡­;
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