| Product Name | TIAL1, 1-375aa, Human |
|---|---|
| Description | TIAL1 is a member of a family of RNA-binding proteins, has three RNA recognition motifs (RRMs), and binds adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control, splicing and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The different isoforms have been show to function differently with respect to post-transcriptional silencing. Recombinant human TIAL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | TIA1 cytotoxic granule-associated RNA binding protein-like 1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR |
| Host | E. coli |
| Molecular Weight | 15.9kDa (139aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMMEDDGQ PRTLYVGNLS RDVTEVLILQ LFSQIGPCKS CKMITEHTSN DPYCFVEFYE HRDAAAALAA MNGRKILGKE VKVNWATTPS SQKKDTSNHF HVFVGDLSPE ITTEDIKSAF APFGKISDAR VVKDMATGKS KGYGFVSFYN KLDAENAIVH MGGQWLGGRQ IRTNWATRKP PAPKSTQENN TKQLRFEDVV NQSSPKNCTV YCGGIASGLT DQLMRQTFSP FGQIMEIRVF PEKGYSFVRF STHESAAHAI VSVNGTTIEG HVVKCYWGKE SPDMTKNFQQ VDYSQWGQWS QVYGNPQQYG QYMANGWQVP PYGVYGQPWN QQGFGVDQSP SAAWMGGFGA QPPQGQAPPP VIPPPNQAGY GMASYQTQ |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Phosphate buffer (pH 8.0) containing 10% glycerol |
| Concentration | 0.25mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Kim HS., et al. (2013) RNA Biol 10 (4), 579-589 Singh NN., et al. (2011) Mol. Cell. Biol. 31 (5), 935-954 |
| Background | TIAL1 is a member of a family of RNA-binding proteins, has three RNA recognition motifs (RRMs), and binds adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control, splicing and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The different isoforms have been show to function differently with respect to post-transcriptional silencing. Recombinant human TIAL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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