| Product Name | TNFa, 80-235aa, Mouse (Bioactivity Validated) |
|---|---|
| Description | TNFa, also known as tumor necrosis factor, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia. Recombinant mouse TNFa protein was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | Tumor necrosis factor, DIF, TNF-alpha, TNFalpha, Tnfsf1a, TNFSF2, Cachectin |
| Host | E. coli |
| Molecular Weight |
80.9kDa (708aa) 70-100kDa (SDS-PAGE under reducing conditions) |
| Amino Acid Sequence | MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL |
| Reactivity | Mouse |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl buffer (pH 8.0) containing 100mM NaCl, 0.1mM PMSF, 10% glycerol. |
| Concentration | 1mg/ml (determined by Absorbance at 280nm) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Shakhov A.N., et al. (1987) Bioorg. Khim. 13:701-705 Pennica D., et al. (1985) Proc. Natl. Acad. Sci. U.S.A. 82:6060-6064 |
| Background | TNFa, also known as tumor necrosis factor, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia. Recombinant mouse TNFa protein was expressed in E.coli and purified by using conventional chromatography. |
| Supplier | ARP |
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