| Product Name | TNFa CLIA kit (Rat) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Alpha (TNFa). No significant cross-reactivity or interference between Tumor Necrosis Factor Alpha (TNFa) and analogues was observed. |
| Synonyms | DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2 |
| Method | Sandwich |
| Detection Range | 1.37-1,000pg/mL |
| Sensitivity | 0.50pg/mL |
| Target | TNFa |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Alpha (TNFa). No significant cross-reactivity or interference between Tumor Necrosis Factor Alpha (TNFa) and analogues was observed. |
| Reactivity | Rat |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 100uL Substrate Solution.Incubate 10 minutes at 37℃; 8.Read RLU value immediately. |
| Uniprot | P16599 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Chemiluminescence |
| References | Swimming training attenuates the morphological reorganization of the myocardium and local inflammation in the left ventricle of growing rats with untreated experimental diabetes.;Uroprotective effect of oleuropein in a rat model of hemorrhagic cystitis.;Bone fracture healing is delayed in splenectomic rats;Learning and memory improvement and neuroprotection of Gardenia jasminoides (Fructusgardenia) extract on ischemic brain injury rats.;Protective effects of N-acetylcystein and atorvastatin against renal and hepatic injury in a rat model of intestinal ischemia-reperfusion;Impact of purified oat 1-3, 1-4-β-d-glucan of different molecular weight on alleviation of inflammation parameters during gastritis;Effect of boron on thymic cytokine expression, hormone secretion, antioxidant functions, cell proliferation, and apoptosis potential via the ERK1/2 signaling pathway;Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1);Protective effect of Vitamin D3 against lead induced hepatotoxicity, oxidative stress, immunosuppressive and calcium homeostasis disorders in rat;Th17*;Activation of the Peroxisome Proliferator-Activated Receptors (PPAR-α/γ) and the Fatty Acid Metabolizing Enzyme Protein CPT1A by Camel Milk Treatment …;Photobiomodulation associated with lipid nanoparticles and hyaluronic acid accelerate the healing of excisional wounds;Pretreatment with Shenmai Injection Protects against Coronary Microvascular Dysfunction; |
| Supplier | Cloud Clone |
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