| Product Name | TPSAB1, 31-275aa, Human, Baculovirus |
|---|---|
| Description | TPSAB1, also known as tryptase alpha/beta-1, is a serine protease with trypsin-like specificity. It is the key neutral protease present in mast cells and is discharged upon the coupled activation-degranulation response of this cell type. This protein is enzymatically active only as a heparin-stabilized tetramer, and is resistant to all known endogenous proteinase inhibitors. Also, it is implicated as a mediator in the pathogenesis of asthma and other allergic and inflammatory disorders. Recombinant human TPSAB1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Synonyms | Tryptase alpha/beta-1, TPSAB1, TPS1, TPS2, TPSB1 |
| Host | Virus |
| Molecular Weight |
34.1kDa (298aa) 28-40KDa (SDS-PAGE under reducing conditions.) |
| Amino Acid Sequence | IVGGQEAPRS KWPWQVSLRV HGPYWMHFCG GSLIHPQWVL TAAHCVGPDV KDLAALRVQL REQHLYYQDQ LLPVSRIIVH PQFYTAQIGA DIALLELEEP VNVSSHVHTV TLPPASETFP PGMPCWVTGW GDVDNDERLP PPFPLKQVKV PIMENHICDA KYHLGAYTGD DVRIVRDDML CAGNTRRDSC QGDSGGPLVC KVNGTWLQAG VVSWGEGCAQ PNRPGIYTRV TYYLDWIHHY VPKKPHHHHH H |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in Phosphate Buffered Saline (pH 7.4) containing 40% glycerol, 1mM DTT. |
| Concentration | 0.25mg/ml (determined by Absorbance at 280nm) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Schwartz,L.B., et al, (1985) J. Immunol. 135:2762-2767. Lewicki L., et al, (2015) Mediators Inflamm. 2015:395173. |
| Background | TPSAB1, also known as tryptase alpha/beta-1, is a serine protease with trypsin-like specificity. It is the key neutral protease present in mast cells and is discharged upon the coupled activation-degranulation response of this cell type. This protein is enzymatically active only as a heparin-stabilized tetramer, and is resistant to all known endogenous proteinase inhibitors. Also, it is implicated as a mediator in the pathogenesis of asthma and other allergic and inflammatory disorders. Recombinant human TPSAB1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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