| Product Name | TPST1, 26-370aa, Human (Denatured) |
|---|---|
| Description | TPST1 also known as protein-tyrosine sulfotransferase 1. Tyrosylprotein Sulfotransferase is the enzyme that catalyzes the sulfation reaction of protein tyrosines, a post-translational modification of proteins. It utilizes 3'-Phosphoadenosine-5'-phosphosulfate (PAPS) as the sulfonate donor and binds proteins with target tyrosine residues to eventually form the tyrosine O-sulfate ester group and the desulfonated 3'-phosphoadenosine-5'-phosphate (PAP). Recombinant human TPST1 fused to His-tag at N-terminus, was expressed in E.coli. |
| Synonyms | Protein-tyrosine sulfotransferase 1, Protein-tyrosine sulfotransferase 1, TANGO13A |
| Host | E. coli |
| Molecular Weight | 69.8kDa (645aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH TGSMQHAMEC HHRIEERSQP VKLESTRTTV RTGLDLKANK TFAYHKDMPL IFIGGVPRSG TTLMRAMLDA HPDIRCGEET RVIPRILALK QMWSRSSKEK IRLDEAGVTD EVLDSAMQAF LLEIIVKHGE PAPYLCNKDP FALKSLTYLS RLFPNAKFLL MVRDGRASVH SMISRKVTIA GFDLNSYRDC LTKWNRAIET MYNQCMEVGY KKCMLVHYEQ LVLHPERWMR TLLKFLQIPW NHSVLHHEEM IGKAGGVSLS KVERSTDQVI KPVNVGALSK WVGKIPPDVL QDMAVIAPML AKLGYDPYAN PPNYGKPDPK IIENTRRVYK GEFQLPDFLK EKPQTEQVE |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl (pH8.0) containing 10% glycerol |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Ouyang Y.-B.,et al. (1998) roc. Natl. Acad. Sci. u.S.A. 95:2896-2901 |
| Background | TPST1 also known as protein-tyrosine sulfotransferase 1. Tyrosylprotein Sulfotransferase is the enzyme that catalyzes the sulfation reaction of protein tyrosines, a post-translational modification of proteins. It utilizes 3'-Phosphoadenosine-5'-phosphosulfate (PAPS) as the sulfonate donor and binds proteins with target tyrosine residues to eventually form the tyrosine O-sulfate ester group and the desulfonated 3'-phosphoadenosine-5'-phosphate (PAP). Recombinant human TPST1 fused to His-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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