| Product Name | TPST2, 26-377aa, Human, Baculovirus |
|---|---|
| Description | TPST2, also known as protein-tyrosine sulfotransferase 2, is the enzyme that catalyzes the sulfation reaction of protein tyrosines, a post-translational modification of proteins. This protein is a type II integral membrane protein found in the Golgi body. Compared to TPST1, TPST2 has similar tissue distribution and overlapping substrate specificity. In contrast to TPST1-/- males with normal fertility in mice, TPST2-/- males are infertile due to severe defects in sperm motility. Recombinant human TPST2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Synonyms | Protein-tyrosine sulfotransferase 2, TANGO13B |
| Host | Virus |
| Molecular Weight |
26.4kDa (232aa) 28-40KDa (SDS-PAGE under reducing conditions.) |
| Amino Acid Sequence | ADPQQVLECR AVLAGLRSPR GAMRPEQEEL VMVGTNHVEY RYGKAMPLIF VGGVPRSGTT LMRAMLDAHP EVRCGEETRI IPRVLAMRQA WSKSGREKLR LDEAGVTDEV LDAAMQAFIL EVIAKHGEPA RVLCNKDPFT LKSSVYLSRL FPNSKFLLMV RDGRASVHSM ITRKVTIAGF DLSSYRDCLT KWNKAIEVMY AQCMEVGKEK CLPVYYEQLV LHPRRSLKLI LDFLGIAWSD AVLHHEDLIG KPGGVSLSKI ERSTDQVIKP VNLEALSKWT GHIPGDVVRD MAQIAPMLAQ LGYDPYANPP NYGNPDPFVI NNTQRVLKGD YKTPANLKGY FQVNQNSTSS HLGSSHHHHH H |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol. |
| Concentration | 0.5mg/ml (determined by Absorbance at 280nm) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Danan LM., et al. (2008). J Am Soc Mass Spectrom. 19(10):1459-1466. Kehoe J.W. and Bertozzi C.R. (2000) Chemistry & Biology. 7:57-61. |
| Background | TPST2, also known as protein-tyrosine sulfotransferase 2, is the enzyme that catalyzes the sulfation reaction of protein tyrosines, a post-translational modification of proteins. This protein is a type II integral membrane protein found in the Golgi body. Compared to TPST1, TPST2 has similar tissue distribution and overlapping substrate specificity. In contrast to TPST1-/- males with normal fertility in mice, TPST2-/- males are infertile due to severe defects in sperm motility. Recombinant human TPST2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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