| Product Name | Trypsin-3, 81-304aa, Human, Baculovirus |
|---|---|
| Description | PRSS3, also known as trypsin-3, is a member of the trypsin family of serine proteases. It is specialized for the degradation of trypsin inhibitors and may be involved in defensin processing, including DEFA5. This protein is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Compared to PRSS1 and 2, one intriguing feature of PRSS3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. Recombinant human PRSS3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Synonyms | PRSS3, MTG, PRSS4, T9, TRY3, TRY4 |
| Host | Virus |
| Molecular Weight |
15.6kDa (145aa) 18-28KDa (SDS-PAGE under reducing conditions.) |
| Amino Acid Sequence | ADPIVGGYTC EENSLPYQVS LNSGSHFCGG SLISEQWVVS AAHCYKTRIQ VRLGEHNIKV LEGNEQFINA AKIIRHPKYN RDTLDNDIML IKLSSPAVIN ARVSTISLPT APPAAGTECL ISGWGNTLSF GADYPDELKC LDAPVLTQAE CKASYPGKIT NSMFCVGFLE GGKDSCQRDS GGPVVCNGQL QGVVSWGHGC AWKNRPGVYT KVYNYVDWIK DTIAANSHHH HHH |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol. |
| Concentration | 0.5mg/ml (determined by Absorbance at 280nm) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Jiang G., et al. (2010) Gut 59(11):1535-1544. Rosendahl J., et al. (2010) Pancreatology 10(2-3):243-249. |
| Background | PRSS3, also known as trypsin-3, is a member of the trypsin family of serine proteases. It is specialized for the degradation of trypsin inhibitors and may be involved in defensin processing, including DEFA5. This protein is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Compared to PRSS1 and 2, one intriguing feature of PRSS3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. Recombinant human PRSS3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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