| Product Name | TYRP1, 25-477aa, Human, Baculovirus |
|---|---|
| Description | TYRP1, also known as 5, 6-dihydroxyindole-2-carboxylic acid oxidase, is a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. It is a melanocyte-specific gene product involved in eumelanin synthesis. It is involved in the oxidation of 5,6-dihydroxyindole-2-carboxylic acid(DHICA) into indole-5,6-quinone-2-carboxylic acid. It is regulated by the microphthalmia-associated transcription factor(MITF). Recombinant human TYRP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Synonyms | 5, 6-dihydroxyindole-2-carboxylic acid oxidase, TYRP1, b-PROTEIN, CAS2, CATB, GP75, OCA3, TRP, TRP1, TYRP |
| Host | Virus |
| Molecular Weight | 36.4 kDa(333aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | ADLQFPRQCA TVEALRSGMC CPDLSPVSGP GTDRCGSSSG RGRCEAVTAD SRPHSPQYPH DGRDDREVWP LRFFNRTCHC NGNFSGHNCG TCRPGWRGAA CDQRVLIVRR NLLDLSKEEK NHFVRALDMA KRTTHPLFVI ATRRSEEILG PDGNTPQFEN ISIYNYFVWT HYYSVKKTFL GVGQESFGEV DFSHEGPAFL TWHRYHLLRL EKDMQEMLQE PSFSLPYWNF ATGKNVCDIC TDDLMGSRSN FDSTLISPNS VFSQWRVVCD SLEDYDTLGT LCNSTEDGPI RRNPAGNVAR PMVQRLPEPQ DVAQCLEVGL FDTPPFYSNS TNSFRNTVEG YSDPTGKYDP AVRSLHNLAH LFLNGTGGQT HLSPNDPIFV LLHTFTDAVF DEWLRRYNAD ISTFPLENAP IGHNRQYNMV PFWPPVTNTE MFVTAPDNLG YTYEIQWPSR EFSVPEHHHH HH |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Sarangarajan R., et al. (2001) Pigment Cell Res. 14:437-444. Chalupa A., et al. (2015) J Invest Dermatol. 135:202-211. |
| Background | TYRP1, also known as 5, 6-dihydroxyindole-2-carboxylic acid oxidase, is a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. It is a melanocyte-specific gene product involved in eumelanin synthesis. It is involved in the oxidation of 5,6-dihydroxyindole-2-carboxylic acid(DHICA) into indole-5,6-quinone-2-carboxylic acid. It is regulated by the microphthalmia-associated transcription factor(MITF). Recombinant human TYRP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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