| Product Name | Ubc7 (Ubiquitin Conjugating enzyme 7) Human Recombinant |
|---|---|
| Description | Human Ubquitin-conjugating enzyme 7(UbcH7) is a ubiquitin-conjugating enzyme (E2) mediating c-fos degradation, transcription factor NF-kappa B maturation, and human papilloma virus-mediated p53 and Myc protein degradation, in vitro. The ubiquitin-conjugating enzymes(E2s) are essential components of the post-translational protein ubiquitination pathway, mediating the transfer of activated ubiquitin to substrate proteins. The human UBE2L1-UBE2L4 gene could potentially encode different isoforms of the UbcH7. UBE2L3 gene, located at chromosome 22q11.2, is the only identical family member with introns and encodes a polypeptide sequence identical to that of UbcH7. UbcH7(154amino acid) was over-expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | UBE2G1, UBE2G, Ubc7 (Ubiquitin Conjugating enzyme 7), EC 6.3.2.19, Ubiquitin-protein ligase G1, Ubiquitin carrier protein G1, E217K, UBC7, Ubiquitin-conjugating enzyme E2 G1, Ubiquitin-conjugating enzyme E2L 3 UBC 7, UBC7 homolog yeast, UBE 2G, Ubiquitin conjugating enzyme E2 G1, Ubiquitin conjugating enzyme E2G 1 (homologous to C, elegans UBC7), Ubiquitin conjugating enzyme E2G 1 (UBC7 homolog C, elegans) antibody, Ubiquitin conjugating enzyme E2G 1 (UBC7 homolog yeast), Ubiquitin conjugating enzyme E2G 1, Ubiquitin protein ligase G1, |
| Host | E. coli |
| Molecular Weight | 23 kDa (211 aa) |
| Amino Acid Sequence | MAASRRLMKE LEEIRKCGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA FRIEINFPAE YPFKPPKITF KTKIYHPNID EKGQVCLPVI SAENWKPATK TDQVIQSLIA LVNDPQPEHP LRADLAEEYS KDRKKFCKNA EEFTKKYGEK RPVD |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris pH 7.5, 300mM NaCl, 0.1mM DTT, 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Ardley HC., et al. (2000) Biochim Biophys Acta. 1491(1-3):57-64. Moynihan TP., et al. (1998) Genomics. 51(1):124-127. Nuber U., et al (1996) J Biol Chem. 271(5):2795-2800 |
| Background | Human Ubquitin-conjugating enzyme 7(UbcH7) is a ubiquitin-conjugating enzyme (E2) mediating c-fos degradation, transcription factor NF-kappa B maturation, and human papilloma virus-mediated p53 and Myc protein degradation, in vitro. The ubiquitin-conjugating enzymes(E2s) are essential components of the post-translational protein ubiquitination pathway, mediating the transfer of activated ubiquitin to substrate proteins. The human UBE2L1-UBE2L4 gene could potentially encode different isoforms of the UbcH7. UBE2L3 gene, located at chromosome 22q11.2, is the only identical family member with introns and encodes a polypeptide sequence identical to that of UbcH7. UbcH7(154amino acid) was over-expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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