| Product Name | Ubc9 (Human ubiquitin conjugating enzyme 9) Human, Recombinant |
|---|---|
| Description | Human Ubc9 is homologous to ubiquitin-conjugating enzymes(E2s). However, instead of conjugating ubiquitin, it conjugates a ubiquitin homologue, small ubiquitin-like modifier 1(SUMO-1). And hUbc9 retains striking structural and functional conservation with yeast Ubc9. The ubiquitin-dependent protein degradation system has been recognized as a complete enzymatic pathway that is responsible for the selective degradation of abnormal and short-lived proteins. The conjugation of ubiquitin reuires the activities of ubiquitin-activating(E1) and -conjugating(E2) enzymes. |
| Synonyms | UBE2I, UBC9, UBCE9, Ubc9, Human ubiquitin conjugating enzyme 9, EC 6.3.2, SUMO-protein ligase, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, Ubiquitin carrier protein I, Ubiquitin carrier protein 9, p18, SUMO-conjugating enzyme UBC9, Ubiquitin-conjugating enzyme E2I C358B7.1, Homologous to yeast UBC9, p18, SUMO 1 conjugating enzyme, SUMO 1 protein ligase, SUMO conjugating enzyme UBC9, SUMO protein ligase, SUMO1 conjugating enzyme, SUMO1 protein ligase, UBC 9, UBC9 homolog, UBCE 9, UBE 2I, Ubiquitin carrier protein, Ubiquitin conjugating enzyme 9, Ubiquitin conjugating enzyme E2I, Ubiquitin conjugating enzyme UbcE2A, Ubiquitin like protein SUMO 1 conjugating enzyme, Ubiquitin like protein SUMO1 conjugating enzyme, Ubiquitin protein ligase E2I, Ubiquitin protein ligase I, |
| Host | E. coli |
| Molecular Weight | 16.8 kDa (151 aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA QAKKFAPS |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in PBS (pH 7.4) |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Tatham MH., et al (2003) Biochemistry. 42(11) 3168-3179 Donghai Lin, et al (2002) J. Biol. Chem. 277(24) 21740-21748. Wang ZY., et al. (1996) J. Biol. Chem. 271(40):24811-6. |
| Background | Human Ubc9 is homologous to ubiquitin-conjugating enzymes(E2s). However, instead of conjugating ubiquitin, it conjugates a ubiquitin homologue, small ubiquitin-like modifier 1(SUMO-1). And hUbc9 retains striking structural and functional conservation with yeast Ubc9. The ubiquitin-dependent protein degradation system has been recognized as a complete enzymatic pathway that is responsible for the selective degradation of abnormal and short-lived proteins. The conjugation of ubiquitin reuires the activities of ubiquitin-activating(E1) and -conjugating(E2) enzymes. |
| Supplier | ARP |
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