| Product Name | UCHL3, 1-230aa, Human |
|---|---|
| Description | Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCHL3, is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. UCHL3 play a role in the regulation of neuronal development and spermatogenesis and have been implicated in neurodegenerative diseases. UCHL3 protein is 54% identical to that of UCHL1. Recombinant human UCHL3 protprot, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Ubiquitin carboxyl-terminal hydrolase isozyme L3, UCH-L3, Ubiquitin thioesterase L3 |
| Host | E. coli |
| Molecular Weight | 27.4kDa (246aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol, 2mM DTT, 200mM NaCl |
| Concentration | 0.5mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Kwon J., et al. (2007) Exp Anim. 56:71-7. Leroy E., et al. (1998) Nature. 395:451-2. |
| Background | Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCHL3, is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. UCHL3 play a role in the regulation of neuronal development and spermatogenesis and have been implicated in neurodegenerative diseases. UCHL3 protein is 54% identical to that of UCHL1. Recombinant human UCHL3 protprot, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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