| Product Name | VOPP1, 82-172aa, Human |
|---|---|
| Description | Vesicular, overexpressed in cancer, prosurvival protein 1(VOPP1), also known as Glioblastoma-amplified secreted protein (GASP), has been previously shown to be over-expressed in human glioblastoma multiforme and squamous cell carcinoma. VOPP1 is a key regulator of NF-kappaB signaling, and that high-level, amplification-mediated VOPP1 expression, such as that occurring in tumors with amplified EGFR, could contribute to resistance to apoptosis. Recombinant human VOPP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Vesicular, overexpressed in cancer, prosurvival protein 1, ECOP, GASP |
| Host | E. coli |
| Molecular Weight | 18.8kDa (160aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MRRRMYPPPL IEEPAFNVSY TRQPPNPGPG AQQPGPPYYT DPGGPGMNPV GNSMAMAFQV PPNSPQGSVA CPPPPAYCNT PPPPYEQVVK AK |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100mM NaCl |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Park S., et al. (2005) Oncogene. 24(15):2495-502. Barse A., et al. (2010) J Mol Histol. 41(2):153-64. |
| Background | Vesicular, overexpressed in cancer, prosurvival protein 1(VOPP1), also known as Glioblastoma-amplified secreted protein (GASP), has been previously shown to be over-expressed in human glioblastoma multiforme and squamous cell carcinoma. VOPP1 is a key regulator of NF-kappaB signaling, and that high-level, amplification-mediated VOPP1 expression, such as that occurring in tumors with amplified EGFR, could contribute to resistance to apoptosis. Recombinant human VOPP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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