| Product Name | VPS25, 1-176aa, Human |
|---|---|
| Description | VPS25, also known as vacuolar protein-sorting-associated protein 25, consists of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may play a role in transcription regulation, possibly via its interaction with ELL. Recombinant human VPS25 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Vacuolar protein-sorting-associated protein 25, DERP9, EAP20, FAP20 |
| Host | E. coli |
| Molecular Weight | 30.2kDa (277aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMAMSFE WPWQYRFPPF FTLQPNVDTR QKQLAAWCSL VLSFCRLHKQ SSMTVMEAQE SPLFNNVKLQ RKLPVESIQI VLEELRKKGN LEWLDKSKSS FLIMWRRPEE WGKLIYQWVS RSGQNNSVFT LYELTNGEDT EDEEFHGLDE ATLLRALQAL QQEHKAEIIT VSDGRGVKFF |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 50% glycerol, 300mM NaCl, 2mM EDTA |
| Concentration | 0.25mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Bailey,S.D. et al. (2010) Diabetes Care 33 (10), 2250-2253 Talmud,P.J. et al. (2009) Am. J. Hum. Genet. 85 (5), 628-642 |
| Background | VPS25, also known as vacuolar protein-sorting-associated protein 25, consists of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may play a role in transcription regulation, possibly via its interaction with ELL. Recombinant human VPS25 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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