| Product Name | VPS26A, 1-327aa, Human |
|---|---|
| Description | VPS26A, also known as vacuolar protein sorting-associated protein 26A, is essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also this protein is required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Recombinant human VPS26A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | Vacuolar protein sorting-associated protein 26A, HB58; Hbeta58; PEP8A; VPS26 |
| Host | E. coli |
| Molecular Weight | 14.7kDa (125aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMSFLGGF FGPICEIDIV LNDGETRKMA EMKTEDGKVE KHYLFYDGES VSGKVNLAFK QPGKRLEHQG IRIEFVGQIE LFNDKSNTHE FVNLVKELAL PGELTQSRSY DFEFMQVEKP YESYIGANVR LRYFLKVTIV RRLTDLVKEY DLIVHQLATY PDVNNSIKME VGIEDCLHIE FEYNKSKYHL KDVIVGKIYF LLVRIKIQHM ELQLIKKEIT GIGPSTTTET ETIAKYEIMD GAPVKGESIP IRLFLAGYDP TPTMRDVNKK FSVRYFLNLV LVDEEDRRYF KQQEIILWRK APEKLRKQRT NFHQRFESPE SQASAEQPEM |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl buffer, pH 8.0, 20% glycerol, 1mM DTT, 200mM NaCl |
| Concentration | 0.5mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Verges M., et al. (2004) Nat. Cell Biol. 6:763-769 Gullapalli A., et al. (2006) Mol. Biol. Cell. 17:1228-1238 |
| Background | VPS26A, also known as vacuolar protein sorting-associated protein 26A, is essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also this protein is required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Recombinant human VPS26A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Supplier | ARP |
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