| Product Name | VPS28, 1-221aa, Human, Recombinant |
|---|---|
| Description | VPS28 is involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. This protein is a subunit of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. VSP28 binding with the TSG101 may play important roles in budding of Retroviruses, such as HIV-1 and Ebola virus. Recombinant human VPS28 was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | ESCRT-I complex subunit VPS28, H-Vps28, Vacuolar Protein Sorting 28 acuolar protein sorting 28, Vacuolar protein sorting 28 (yeast), Vacuolar protein sorting 28 homolog. |
| Host | E. coli |
| Molecular Weight | 22.4 kDa (203aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ VRQMLFDLES AYNAFNRFLH A |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol, 2mM EDTA |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Woodman P., et al. (2001) J Biol Chem. 276(15):11735-42. Bieniasz PD., et al. (2003) J Virol. 77(8):4794-804. |
| Background | VPS28 is involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. This protein is a subunit of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. VSP28 binding with the TSG101 may play important roles in budding of Retroviruses, such as HIV-1 and Ebola virus. Recombinant human VPS28 was expressed in E.coli and purified by using conventional chromatography. |
| Supplier | ARP |
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