| Product Name | Wif-1, 29-379aa, Human, Recombinant, Hi-5 cell |
|---|---|
| Description | Wnt inhibitory factor 1, also known as Wif-1, is a secreted protein that binds to Wnt proteins involved in the embryonic development and cancer, and inhibits their activity. Wif-1 protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals. Recombinant human Wif-1 protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography. |
| Synonyms | Wnt inhibitory factor 1, WIF1, Wnt inhibitory factor 1 |
| Host | E. coli |
| Molecular Weight | 17.3 kDa (152 aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | ADLGPPQEES LYLWIDAHQA RVLIGFEEDI LIVSEGKMAP FTHDFRKAQQ RMPAIPVNIH SMNFTWQAAG QAEYFYEFLS LRSLDKGIMA DPTVNVPLLG TVPHKASVVQ VGFPCLGKQD GVAAFEVDVI VMNSEGNTIL KTPQNAIFFK TCQQAECPGG CRNGGFCNER RICECPDGFH GPHCEKALCT PRCMNGGLCV TPGFCICPPG FYGVNCDKAN CSTTCFNGGT CFYPGKCICP PGLEGEQCEI SKCPQPCRNG GKCIGKSKCK CSKGYQGDLC SKPVCEPGCG AHGTCHEPNK CQCQEGWHGR HCNKRYEASL IHALRPAGAQ LRQHTPSLKK AEERRDPPES NYIWSGRLVP RGSHHHHHH |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | >80 % by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Wissmann C. et al. (2003) J. Pathol. 201:204-12 Cebrat M. et al. (2004) Cancer Lett. 206:107-13 |
| Background | Wnt inhibitory factor 1, also known as Wif-1, is a secreted protein that binds to Wnt proteins involved in the embryonic development and cancer, and inhibits their activity. Wif-1 protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals. Recombinant human Wif-1 protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography. |
| Supplier | ARP |
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