| Product Name | ZWINT, 1-230aa, Human |
|---|---|
| Description | ZW10 interactor isoform b, also known as ZWINT, is clearly involved in kinetochore function. Localized to the cytoplasm during interphase and to kinetochores from late prophase to anaphase, ZWINT interacts with ZW10 (Zeste White 10) and functions to regulate the association between ZW10 and kinetochores. Defects in the gene encoding ZWINT are associated with the pathogenesis of Roberts’s syndrome, an autosomal recessive disorder characterized by growth retardation due to premature chromosome separation. Recombinant human ZWINT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | ZW10 interactor isoform b, HZwint-1, KNTC2AP, ZWINT1 |
| Host | E. coli |
| Molecular Weight | 18.1 kDa (158 aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMEAAETE AEAAALEVLA EVAGILEPVG LQEEAELPAK ILVEFVVDSQ KKDKLLCSQL QVADFLQNIL AQEDTAKGLD PLASEDTSRQ KAIAAKEQWK ELKATYREHV EAIKIGLTKA LTQMEEAQRK RTQLREAFEQ LQAKKQMAME KRRAVQNQWQ LQQEKHLQHL AEVSAEGKLL FPEAEAEAEN LPDDKPQQPT RPQEQSTGDT MGRDPGVSFK AVGLQPAGDV NLP |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea |
| Concentration | 1.0 mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Musio A., et al. (2004) Gene. 331: 33-40. Lin Y T., et al. (2006) Oncogene. 25: 6901-6914. |
| Background | ZW10 interactor isoform b, also known as ZWINT, is clearly involved in kinetochore function. Localized to the cytoplasm during interphase and to kinetochores from late prophase to anaphase, ZWINT interacts with ZW10 (Zeste White 10) and functions to regulate the association between ZW10 and kinetochores. Defects in the gene encoding ZWINT are associated with the pathogenesis of Roberts’s syndrome, an autosomal recessive disorder characterized by growth retardation due to premature chromosome separation. Recombinant human ZWINT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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