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This is the first post in a new series looking at ELISA troubleshooting tips – Poor standard curve ELISAs, generally, are pretty straightforward assays to carry […]

Sandwich ELISA This is the third post in a series on ELISA, and this one will focus on the Sandwich ELISA. A sandwich ELISA differs from […]

Indirect ELISA This is the third post in our series of posts and protocols on Enzyme Linked Immunosorbent Assays – ELISAs. The first was a detailed […]

How to evenly load protein on western blots (protein quantitation for western blots) A key point in performing a good and reliable western blot is to […]

Direct ELISA This is the second in a series of posts and protocols on Enzyme Linked Immunosorbent Assays (ELISAs). This one deals with the ‘Direct ELISA’. […]

ELISA If you need to measure the concentration or presence of an antigen in solution, then the Enzyme Linked Immunosorbent Assay (ELISA) is one of the workhorses […]

Immunohistochemistry on Frozen tissues IHC Protocol – Frozen Tissue: An introduction This is the second post in a series on immunohistochemistry (IHC). The first post looked at […]

IHC Protocol – paraffin embedded tissues Introduction to IHC-P Put simply, immunohistochemistry (IHC) is a way of staining tissue samples to reveal if and where a specific […]

Synaptopodin Background Synaptopodin is an actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot […]

Non-Specific Bands on your Western Blot Sometimes when you look at your blot everything looks pretty much good. A nice strong band where you’re expecting it, […]