Blog

This is the third in a series of blog posts looking at troubleshooting problems with ELISAs. This one is going to focus on the possible causes […]

This is the second in a series of blog posts looking at troubleshooting problems with ELISAs. This one is going to focus on the causes and […]

This is the first post in a new series looking at ELISA troubleshooting tips – Poor standard curve ELISAs, generally, are pretty straightforward assays to carry […]

Sandwich ELISA This is the third post in a series on ELISA, and this one will focus on the Sandwich ELISA. A sandwich ELISA differs from […]

Indirect ELISA This is the third post in our series of posts and protocols on Enzyme Linked Immunosorbent Assays – ELISAs. The first was a detailed […]

How to evenly load protein on western blots (protein quantitation for western blots) A key point in performing a good and reliable western blot is to […]

Direct ELISA This is the second in a series of posts and protocols on Enzyme Linked Immunosorbent Assays (ELISAs). This one deals with the ‘Direct ELISA’. […]

ELISA If you need to measure the concentration or presence of an antigen in solution, then the Enzyme Linked Immunosorbent Assay (ELISA) is one of the workhorses […]

Immunohistochemistry on Frozen tissues IHC Protocol – Frozen Tissue: An introduction This is the second post in a series on immunohistochemistry (IHC). The first post looked at […]

IHC Protocol – paraffin embedded tissues Introduction to IHC-P Put simply, immunohistochemistry (IHC) is a way of staining tissue samples to reveal if and where a specific […]