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Western blot troubleshooting: no signal
Western blot: No signal… Perhaps the biggest and most frustrating issue with a western blot is when you’ve finished everything, and…there’s nothing. No signal at all. […]
Western blot troubleshooting: Multiple bands
What it means when you get multiple bands on your western blot You’ve run the blot. Now it sits in front of you, and looks more […]
Western blot troubleshooting: high background
Common causes of high background in Western Blotting When your blot comes out looking like a bad x-ray, you’ve got background problems. It’s the result of your […]
Dot to dot: Troubleshooting spotty or patchy western blots
Dark spots and white patches on western blot You came in early today, as you really needed the results of this western blot. It’ll determine what […]
Western Blot Protocol
A Simple Western Blot Protocol from ARP At its core, a western blot (or ‘immunoblot’) is simply a way of detecting if a specific protein is present […]
Monoclonal Antibodies vs Polyclonal Antibodies
Monoclonal Antibodies vs Polyclonal Antibodies: what are the differences and how they will affect your experiments Monoclonal antibodies vs Polyclonal antibodies: The different production processes Both polyclonal […]
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