Blogs

ELISA If you need to measure the concentration or presence of an antigen in solution, then the Enzyme Linked Immunosorbent Assay (ELISA) is one of the workhorses […]

Immunohistochemistry on Frozen tissues IHC Protocol – Frozen Tissue: An introduction This is the second post in a series on immunohistochemistry (IHC). The first post looked at […]

IHC Protocol – paraffin embedded tissues Introduction to IHC-P Put simply, immunohistochemistry (IHC) is a way of staining tissue samples to reveal if and where a specific […]

Synaptopodin Background Synaptopodin is an actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot […]

Non-Specific Bands on your Western Blot Sometimes when you look at your blot everything looks pretty much good. A nice strong band where you’re expecting it, […]

Western blot: No signal… Perhaps the biggest and most frustrating issue with a western blot is when you’ve finished everything, and…there’s nothing. No signal at all. […]

What it means when you get multiple bands on your western blot You’ve run the blot. Now it sits in front of you, and looks more […]

Common causes of high background in Western Blotting When your blot comes out looking like a bad x-ray, you’ve got background problems. It’s the result of your […]

Dark spots and white patches on western blot You came in early today, as you really needed the results of this western blot. It’ll determine what […]

A Simple Western Blot Protocol from ARP At its core, a western blot (or ‘immunoblot’) is simply a way of detecting if a specific protein is present […]